A3853
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 | Immunoblotting Chicken fibroblast cells extract was separated on SDS-PAGE and probed with Monoclonal PA to Actin (Cat. No. A3853). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A1293) and a NBT/BCIP substrate. Antibody concentration 0.5 μg/mL. |
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A3853
Sigma
Monoclonal Anti-Actin antibody produced in mouse
clone AC-40, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-Actin |
Description
| Immunogen | synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solutions may also be used as fixatives. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | AC-40 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | goat, viper, Xenopus, bovine, guinea pig, snail, canine, hamster, rat, chicken, mouse, human, sheep, pig, rabbit, carp |
| application(s) | immunoblotting: 1-2 μg/mL using chicken or human fibroblasts |
| immunocytochemistry: 10-20 μg/mL using chicken or human fibroblasts by methanol/acetone fixation | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG2a |
| Gene Information | human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72) |
| rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365) | |
| mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Safety Statements | 23-24/25 |
| WGK Germany | NWG |
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References
| reference | Autret, A., et al., Poliovirus induces Bax-dependent cell death mediated by c-Jun NH2-terminal kinase. J. Virol. 81, 7504-7516, (2007) Abstract |
| Garcia, A., et al., Functional Interaction of Aurora-A and PP2A during Mitosis Mol. Biol. Cell 18, 1233-1241, (2007) Abstract | |
| Choubey, D., et al., Restoration of p53 Expression in Human Cancer Cell Lines Upregulates the Expression of Notch1: Implications for Cancer Cell Fate Determination after Genotoxic Stress1 Neoplasia 9, 427-434, (2007) Abstract | |
| Albiges-Rizo, C., Down regulation of talin alters cell adhesion and processing of the α5β1 integrin. J. Cell Sci. 108, 3317, (1995) |
