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Sigma
Monoclonal Anti-Actin antibody produced in mouse
A3853 -
clone AC-40, purified immunoglobulin, buffered aqueous solution
DOWNLOAD MSDS (PDF)Synonym: Monoclonal Anti-Actin
Properties
| Related Categories | A, AC-AC, Actin Antibodies, Alphabetical Index, Antibodies, |
| antibody form | purified immunoglobulin |
| clone | AC-40, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 42 kDa |
| species reactivity | goat, viper, Xenopus, bovine, guinea pig, snail, canine, hamster, rat, chicken, mouse, human, sheep, pig, rabbit, carp |
| application(s) | immunoblotting: 1-2 μg/mL using chicken or human fibroblasts |
| immunocytochemistry: 10-20 μg/mL using chicken or human fibroblasts by methanol/acetone fixation | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgG2a |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Immunogen
synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-H
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti
Specificity
The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Price and Availability
Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody.
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Documents
Certificate of Analysis
| A3853 - Datasheet (32 KB) |
| Antibody Explorer |  |
| Compatible Secondary Abs |  |
| Similar Products |
Safety Information
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| Safety Statements | 23-24/25 |
| WGK Germany | 2 |
Technical information & documentation associated with this product is available in the Safety & Documentation tab.
Articles
Antibodies to Actin and GAPDH
Actin and myosin are the two major cytoskeletal proteins implicated in cell motility. As constituents of many cell types, actin and myosin are involved in a myriad of cellular process including locom...
Leigh Gaskill
BioFiles 2008, 3.6, 10.
Keywords: Immunofluorescence, PAGE, Western blot
References
Autret, A., et al., Poliovirus induces Bax-dependent cell death mediated by c-Jun NH2-terminal kinase. J. Virol. 81, 7504-7516, (2007) Abstract
Garcia, A., et al., Functional Interaction of Aurora-A and PP2A during Mitosis Mol. Biol. Cell 18, 1233-1241, (2007) Abstract
Choubey, D., et al., Restoration of p53 Expression in Human Cancer Cell Lines Upregulates the Expression of Notch1: Implications for Cancer Cell Fate Determination after Genotoxic Stress1 Neoplasia 9, 427-434, (2007) Abstract
Louneva, N., et al., Caspase-3 Is Enriched In Postsynaptic Densities And Increased In Alzheimer’s Disease Am. J. Pathol. 173, 1488 - 1495, (2008) Abstract
Bennewith, K., et al., The Role Of Tumor Cell–Derived Connective Tissue Growth Factor (CTGF/CCN2) In Pancreatic Tumor Growth Cancer Res. 69, 775 - 784, (2009) Abstract
Lessard, S., et al., Impaired Skeletal Muscle β-Adrenergic Activation And Lipolysis Are Associated With Whole-Body Insulin Resistance In Rats Bred For Low Intrinsic Exercise Capacity Endocrinology 150, 4883 - 4891, (2009) Abstract
Zhen, A., et al., A Single Amino Acid Difference In Human APOBEC3H Variants Determines HIV-1 Vif Sensitivity J. Virol. 84, 1902 - 1911, (2010) Abstract
Zhang, Y., et al., Nuclear Receptor SHP, A Death Receptor That Targets Mitochondria, Induces Apoptosis And Inhibits Tumor Growth Mol. Cell. Biol. 30, 1341 - 1356, (2010) Abstract
Yan, H., et al., Pathways Contributing To Development Of Spontaneous Mammary Tumors In BALB/c-Trp53+/– Mice Am. J. Pathol. 176, 1421 - 1432, (2010) Abstract
Bennewith, K.L., et al., The Role Of Tumor Cell-derived Connective Tissue Growth Factor (CTGF/CCN2) In Pancreatic Tumor Growth. Cancer Res. 69, 775-84, (2009) Abstract
Albiges-Rizo, C., Down regulation of talin alters cell adhesion and processing of the α5β1 integrin. J. Cell Sci. 108, 3317, (1995)
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